a Record control number WOS:000340011900017

a ISSN 0065-1281

a Original cataloging agency NEU GrandLibrary

a Language code of text/sound track or separate title eng

a Classification number QH581

b Item number .C43 2014

a Personal name Ozbey, Ozlem.

a Title Characterization of colony-forming cells in adult human articular cartilage

h Medium Electronic Document

c Statement of responsibility, etc Özlem Özbey, Zeliha Sahin, Nuray Acar, Filiz Tepeköy Özçelik, Alpay Merter Özenci, Sadi Köksoy, İsmail Üstünel.

b Name of publisher, distributor, etc Elsevıer Gmbh,

c Date of publication, distribution, etc 2014.

a Summary, etc Recent studies have shown that adult human articular cartilage contains stem-like cells within the native structure. In this study, we aimed to determine the localization of putative stem cell markers such as CD90, STRO-1, OCT-3/4, CD105 and CD166 in adult human articular cartilage tissue sections and demonstrate the expression of these markers within the expanded surface zone colony-forming (CF) cells and evaluate their differentiation potential. Biopsy samples were either fixed immediately for immunohistochemical analyses or processed for in vitro cell culture. Immunohistochemical and flow cytometly analyses were performed by using CD90, STRO-1, OCT-3/4, CD105 and CD166 antibodies. Isolated colony-forming (CF) cells were further stimulated, by using the appropriate growth factors in their pellet culture, to obtain cartilage, bone and adipose lineages. We observed that the expression of the stem cell markers were in various zones of the human adult cartilage. Flow cytometry results showed that in CF cells the expression of CD90 and CD166 was high, while OCT-3/4 was low. We also determined that CF cells could be stimulated towards cartilage, bone and adipose lineages. The results of this research support the idea that the resident stem-like cells in adult human articular cartilage express these putative stem cell markers, but further experimental investigations are needed to determine the precise localization of these cells. (C) 2014 Elsevier GmbH. All rights reserved.

a Topical term or geographic name as entry element Cell Biology

a Topical term or geographic name as entry element Near East University Article

a Topical term or geographic name as entry element Yakın Doğu Üniversitesi Makale

g Relationship information 2014, Vol.116, Issue:5, p.763-770

x International Standard Serial Number 00651281

a Main entry heading Acta Hıstochemıca

u Uniform Resource Identifier http://library.neu.edu.tr:2048/login?url=http://dx.doi.org/10.1016/j.acthis.2014.01.004